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Improved identification of Pseudomonas savastanoi pv. phaseolicola at the molecular level
Author(s) -
Borowicz B. P.,
Maćkowiak A.,
Pospieszny H.
Publication year - 2002
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1046/j.1365-2338.2002.00590.x
Subject(s) - polymerase chain reaction , oligonucleotide , pathogen , dna , biology , pseudomonas , microbiology and biotechnology , identification (biology) , gene , dna sequencing , bacteria , genetics , botany
Polymerase chain reaction (PCR) technology was used to identify Pseudomonas savastanoi pv. phaseolicola at the DNA level. Oligonucleotide primers were designed on the basis of the DNA sequence of the phaseolotoxin gene cluster of P. s . phaseolicola . Identification of the pathogen was significantly improved and appearance of unspecific bands was greatly diminished by experimentally selecting the most suitable annealing temperature (Tm) value. We obtained a single, strong DNA band of 1.4 kb length, specific for the identification of P. s . phaseolicola , using a PCR programme based on a Tm value of 80 °C.