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p53 Protein expression and proliferative activity in ductal breast carcinomas
Author(s) -
IoakimLiossi A.,
Safioleas M.,
Karakitsos P.,
Athanassiadou P.,
Athanassiades P.,
Gogas J.,
Nakopoulou L.
Publication year - 2000
Publication title -
cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 48
eISSN - 1365-2303
pISSN - 0956-5507
DOI - 10.1046/j.1365-2303.2000.00259.x
Subject(s) - ki 67 , malignancy , immunocytochemistry , pathology , monoclonal antibody , immunohistochemistry , medicine , stage (stratigraphy) , p53 protein , antibody , breast cancer , protein expression , cell , cancer research , cancer , biology , immunology , gene , paleontology , biochemistry , genetics
p53 Protein expression and proliferative activity in ductal breast carcinomas The immunocytochemical expression of p53 protein and Ki‐67 labelling index in tumour cells of 100 ductal breast carcinomas of different histological grade and stage was evaluated in cytological material. In order to investigate p53 expression and Ki‐67 expression an avidin‐extravidin immunocytochemical technique was applied to imprints. Monoclonal antibody (MoAb) DO‐p53 and proliferating cell monoclonal antibody were used as primary antibodies. A statistically significant difference was observed between p53 protein expression and grade of malignancy and clinical stage ( P = 0.001, P < 0.001, respectively). A statistically significant difference was also observed between Ki‐67 LI and histological grade and stage of the tumours ( P < 0.001, P < 0.001 correspondingly). A correlation was observed between p53 protein expression and Ki‐67 LI ( P < 0.001). The immunocytochemical study of p53 protein and Ki‐67 expression in cytological material represents a simple method which can be applied in routine cytological laboratories for the investigation of potential malignancy of ductal breast cancer.