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16.00–16.30 Monday 15 September 2003 7 Papillomavirus testing using Hybrid capture II in a community‐based primary screening setting: preliminary results and potential clinical applications
Author(s) -
Chana P.,
Ktori E.,
Nowicki M.,
Best J.,
Herbert A.,
Kubba A. A.
Publication year - 2003
Publication title -
cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 48
eISSN - 1365-2303
pISSN - 0956-5507
DOI - 10.1046/j.1365-2303.14.s1.1_12.x
Subject(s) - medicine , cytology , triage , obstetrics , colposcopy , abnormality , human papillomavirus , pap smears , gynecology , pathology , cancer , cervical cancer , psychiatry
The aims of this study were (i) to assess the use of Hybrid‐capture II (HCII) alongside conventional cytology for triage of mildly abnormal smears and (ii) to establish the local prevalence of high‐risk human papillomavirus (HRHPV). Study design In an on‐going study, 669 women aged 20–50 were recruited into (i) a study group, with current abnormal cytology or a history of previous abnormal cytology and (ii) an anonymous control group with current negative cytology and no history of abnormal smears. HCII positive was used as a surrogate for mild dyskaryosis in women with negative smears in the study group. Results There was strong correlation between positive HCII tests with degree of cytological abnormality. HCII was positive in 13 of 13 (100%) of women with at least moderate dyskaryosis, 27 of 34 (79%) with mild dyskaryosis, 18 of 36 (50%) with borderline abnormalities, 36 of 250 (14%) of negative smears. In the control group, 50 of 308 (16%) of negative smears were positive. HCII was positive in four of 28 (14%) women with inadequate smears in both groups. HCII was positive at low levels (<10 RLU) in 3% of samples from both groups. No indication of clinical outcome is yet available and also further work is in progress to assess the significance of low‐level positive results. Overall, 148 of 669 (22%) of women were HCII positive. Conclusion HCII can be carried out alongside conventional cytology for the triage of women with mildly abnormal smears. The results suggest a role for HPV testing in re‐assuring women with a past history of abnormal cytology.