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The exon 3‐retaining and the exon 3‐deleted forms of the growth hormone‐binding protein (GHBP) in human serum are regulated differently
Author(s) -
Kratzsch J.,
Wu Z.,
Kiess W.,
Dehmel B.,
BosseHenck A.,
Reuter W.,
Pflaum C. D.,
Strasburger C. J.
Publication year - 2001
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1046/j.1365-2265.2001.01177.x
Subject(s) - growth hormone binding protein , medicine , endocrinology , growth hormone receptor , gene isoform , exon , biology , myostatin , insulin , apolipoprotein b , hormone , growth hormone , muscle hypertrophy , gene , cholesterol , biochemistry
Recently, two isoforms of the growth hormone‐binding protein (GHBP), which is identical with the extracellular domain of the growth hormone receptor (GHR), have been described. One isoform contains the exon 3 (E3(+)GHBP) and one excludes the exon 3 (E3(–)GHBP). The distribution of both isoforms in peripheral blood and their functional relevance is so far unknown. To study the molecular distribution of both species we have analysed sera of 141 subjects with average weight, overweight and obesity by newly developed immunoassays. The relationship between the different molecular forms of GHBP and specific parameters of body composition as well as risk factors of metabolic disturbances, were then examined. The extracellular domain of the exon 3‐retaining and ‐deleted isoforms of the GHR are released as E3(+)GHBP and E3(–)GHBP into the peripheral circulation. Furthermore, both molecular species do not show any correlation to each other ( r = 0·67) and their relative proportion in blood is gender‐dependent with a higher E3(–)GHBP proportion in females ( P < 0·01). E3(+)GHBP appears to have a considerably stronger correlation to indicators (BMI, fat mass, waist circumference) and metabolic risk factors (fasting insulin, uric acid, triglycerides, apolipoprotein B, diastolic blood pressure) of adiposity than E3(–)GHBP, indicating differences in their functional significance. The availability of assays for the determination of GHBP isoforms may be very important for the study of the GH receptor and its soluble extracellular domain, GHBP.