Open AccessAntibody production in autoimmune BXSB mice. I. CD40L‐expressing B cells need fewer signals for polyclonal antibody synthesis
Author(s) -
Sarah J. Blossom,
Kathleen M. Gilbert
Publication year - 1999
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1999.01032.x
Subject(s) - cd40 , antibody , immunology , immunoglobulin d , biology , b cell , immune system , polyclonal antibodies , population , autoimmunity , autoimmune disease , cytokine , b 1 cell , t cell , cytotoxic t cell , antigen presenting cell , medicine , in vitro , biochemistry , environmental health
Male, but not female, BXSB mice develop severe lupus associated with multiple immune system defects. It was recently shown that one immunological abnormality found in male BXSB mice encompasses B cell expression of CD40 ligand (CD40L) by an expanded population of large B cells. The present study was undertaken to determine how the CD40L‐expressing large B cells in male BXSB mice compared with size‐matched B cells from female mice in terms of their ability to secrete antibody. It was shown that the large B cells from female mice, similar to the small B cells from either male or female mice, required CD40 signalling, immunoglobulin cross‐linking and cytokines for optimal antibody synthesis. In contrast, large B cells from male BXSB mice produced high levels of antibody when stimulated with only two of the three signals, and made significantly more total IgM and IgG, and anti‐ssDNA antibody than size‐matched B cells from female mice when stimulated with IL‐4/IL‐5 alone, IL‐4/IL‐5 plus low levels of anti‐IgD‐dextran, or IL‐4/IL‐5 plus anti‐CD40 MoAb. The ability of the large B cells from male mice to produce antibody under suboptimal stimulatory conditions correlated with their expression of CD40L, and was inhibited by CD40‐immunoglobulin. Taken together, these findings suggested that large CD40L‐expressing B cells from male BXSB mice may be able to bypass a need for CD40 signalling from T cells, thus contributing to autoimmune disease by promoting antibody production in the absence of cognate T cell help.