Open AccessContrasting roles for RANTES and macrophage inflammatory protein‐1α (MIP‐1α) in a murine model of allergic peritonitis
Author(s) -
Das A. M.,
Ajuebor M. N.,
Flower R. J.,
Perretti M.,
Mccoll S. R.
Publication year - 1999
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1999.00978.x
Subject(s) - macrophage inflammatory protein , eosinophil , ovalbumin , chemokine , peritoneal cavity , immunology , eotaxin , ccl3 , isotype , allergic inflammation , antibody , ccl11 , inflammation , biology , microbiology and biotechnology , chemistry , antigen , monoclonal antibody , ccl2 , anatomy , asthma
Cell accumulation and CC chemokine production were assessed in the peritoneal cavity of ovalbumin (OVA)‐sensitized mice following antigen challenge. Intraperitoneal challenge with OVA induced a significant eosinophil influx from 6 h post‐challenge with increased numbers persisting at 24 h. At 6 h there was also a marked presence of neutrophils. Messenger RNA expression and protein levels for the chemokines RANTES and MIP‐1α were measured in the cell pellets and supernatants, respectively, from peritoneal washes following OVA challenge. RANTES mRNA was detected from 2 h to 4 h following OVA injection, whereas mRNA for MIP‐1α was only detectable at 4 h. RANTES protein was first detected from 4 h after OVA injection and by 24 h the protein levels had increased further. Basal levels of MIP‐1α were detected in peritoneal washes. These levels peaked at 2 h after OVA challenge and rapidly declined to basal levels by 6 h. A functional role for the chemokines was assessed using neutralizing polyclonal antibodies. Co‐injection of OVA with anti‐RANTES antibodies resulted in a significant inhibition of eosinophil infiltration into the cavity at 6 h and 24 h (63% and 52% inhibition, respectively) without significantly influencing the number of neutrophils present. In contrast, injection of anti‐MIP‐1α antibodies only inhibited neutrophil migration at the 6 h time point by 44% without significantly affecting the accumulation of eosinophils. These results demonstrate an important role for RANTES in mediating eosinophil influx in allergic inflammation and a contrasting role for MIP‐1α in mediating neutrophil recruitment.