Open AccessCytokine‐enhanced mixed lymphocyte reaction (MLR) in cord blood
Author(s) -
Masakazu Umemoto,
Eiichi Azuma,
Masahiro Hirayama,
Marie Nagai,
S Hiratake,
Qi Jiang,
Tadashi Kumamoto,
Michihiro Kobayashi,
Yoshihiro Komada,
Minoru Sakurai
Publication year - 1998
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1998.00607.x
Subject(s) - immunology , mixed lymphocyte reaction , cord blood , cytokine , lymphocyte , medicine , immune system , t cell
Although in cord blood (CB) transplantation graft‐ versus ‐host disease (GVHD) is reported to be less severe, GVHD may occur even in patients with HLA‐identical sibling donors. This result shows that HLA typing can not entirely predict GVHD. The standard MLR with CB cells was either normal or slightly reduced compared with adult peripheral blood (PB) cells. We used two manipulations to increase the responses of CB cells to allo‐antigens. The first was to treat the stimulator cells with cytokines, and the second to amplify weak proliferative responses by adding exogenous cytokines to MLR cultures (modified MLR). The stimulator cells were treated with both interferon‐gamma (IFN‐γ) and IL‐4. The responder cells were treated with both IL‐2 and tumour necrosis factor‐alpha (TNF‐α). It is still to be determined whether or not this cytokine‐enhanced MLR could be a possible predictor of GVHD. However, using these cytokines, 90% of CB could recognize allo‐antigens, even if the standard MLR was negative.