Abnormal T cell responses to bacterial superantigens in Behçet's disease (BD)

This study examines the nature of T cell hypersensitivity in BD. Highly purified T cells from 32 BD patients, from 29 rheumatoid arthritis (RA) patients and from 14 healthy individuals were cultured with various concentrations of Staphylococcal enterotoxins (SE) B and C 1 in the presence of monocytes for 5 days, after which the production of interferon‐gamma (IFN‐γ) was assessed. High concentrations of SE (1 ng/ml) stimulated BD T cells as well as control T cells to produce comparably high amounts of IFN‐γ, whereas low concentrations of SE (1 pg/ml) stimulated BD T cells much more effectively than normal or RA T cells. The hypersensitivity of BD T cells to low concentrations of SEC 1 was restored with RA monocytes instead of BD monocytes, whereas BD monocytes could not elicit the SEC 1 ‐induced IFN‐γ production of RA T cells. Moreover, there were no significant differences between BD T cells and RA T cells in monocyte‐independent IFN‐γ production stimulated with low or high concentrations of immobilized anti‐CD3, or in the monocyte‐mediated enhancement of IFN‐γ production stimulated with a low concentration of immobilized anti‐CD3. These results confirm that T cell hypersensitivity is not confined to certain specific antigens in BD. More importantly, the data strongly suggest that abnormalities in signal transduction triggered by perturbation of T cell receptors, but not in that induced by cross‐linking of CD3 molecules nor in that delivered through costimulation molecules, play an important role in the pathogenesis of BD.