Open AccessComparative study of the complement‐activating and specific IgE‐binding properties of ragweed pollen allergen
Author(s) -
HIDVÉGI T.,
BERRENS L.,
VARGA L.,
MARAÑON F.,
SCHMIDT B.,
KIRSCHFINK M.,
FU¨ST G.
Publication year - 1997
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1997.d01-974.x
Subject(s) - ragweed , immunoglobulin e , allergen , immunology , complement system , biology , chemistry , microbiology and biotechnology , allergy , antibody
Previous reports have defined the capacity of ragweed pollen extract (RWA) to activate human complement (C) in fluid phase through the classical pathway and have ascertained a strong correlation between the extent of complement activation and the severity of symptoms of allergic rhinoconjunctivitis during the ragweed blooming season. In the present study the complement‐activating and specific IgE‐binding capacities of various ragweed allergen preparations were compared. Elimination of physically adsorbed (flavonoid) pigments from the allergenic proteins had no significant effect on their complement‐consuming capacity, although the process strongly diminished specific IgE binding. Removal of an IgE‐binding trypsin inhibitor from RWA significantly enhanced RWA‐induced complement activation, whereas it did not change IgE binding. These findings indicate that neither the physically adsorbed pigments nor the trypsin inhibitor are involved in complement activation by ragweed pollen allergens, and suggest that complement activation and specific IgE binding are distinct molecular properties of ragweed pollen allergen.