Open AccessEstablishment and characterization of cloned CD4 − CD8 − αβ‐T cell receptor (TCR)‐bearing autoreactive T cells from autoimmune NZB×NZW F 1 mice
Author(s) -
CHEN Y.C.,
YE Y.L.,
CHIANG B.L.
Publication year - 1997
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1997.d01-971.x
Subject(s) - immunology , t cell receptor , biology , cd8 , cytotoxic t cell , autoimmune disease , autoimmunity , t cell , receptor , t lymphocyte , immune system , antibody , genetics , in vitro
Murine models such as NZB/W F 1 , NZB.H‐2 bm12 and MRL.lpr/lpr mice have provided greater insight into the pathogenic mechanisms of lupus. To understand further the roles of T cells and cytokines in the pathogenesis of murine lupus, 11 cloned anti‐DNA antibodies augmenting autoreactive T cell lines were derived from NZB/W F 1 mice. All these autoreactive cells responded to syngeneic splenic cells and helped syngeneic B cells to produce anti‐DNA antibodies, especially the IgG antibody. Ten out of 11 autoreactive T cell lines expressed neither CD4 nor CD8 cell surface markers on their surface. In addition, the cytokine production pattern of these autoreactive T cell lines was predominantly of type 0 (Th0) or type 2 T helper cells (Th2). To further investigate the role of accessory molecules in the activation of these autoreactive T cell lines, expression of IL‐2R and heat‐stable antigen (HSA) on these autoreactive T cells was analysed. Results suggest that the HSA played a critical role in the activation and function of these double‐negative cloned autoreactive T cells.