Open AccessThe production of IL‐8 in cerebrospinal fluid in aseptic meningitis of children
Author(s) -
ISHIGURO A.,
SUZUKI Y.,
INABA Y.,
FUKUSHIMA K.,
KOMIYAMA A.,
KOEFFLER H. P.,
SHIMBO T.
Publication year - 1997
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1997.4681366.x
Subject(s) - aseptic meningitis , cerebrospinal fluid , peripheral blood mononuclear cell , meningitis , chemokine , immunology , csf albumin , medicine , granulocyte colony stimulating factor , granulocyte , aseptic processing , pathology , biology , inflammation , in vitro , surgery , biochemistry , chemotherapy
Neutrophils accumulate initially in the cerebrospinal fluid (CSF) of aseptic meningitis, perhaps because of increased levels of granulocyte colony‐stimulating factor (G‐CSF), macrophage inflammatory protein‐1α (MIP‐1α), and IL‐8 in the subarachnoid space. We studied levels of these cytokines in children with aseptic meningitis using ELISA. When meningeal symptoms existed, IL‐8 levels (1399 ± 1600 ng/ l , n = 32) in the CSF were significantly higher than those either after meningeal symptoms disappeared (61 ± 56 ng/ l , n = 18) or in controls (44 ± 63 ng/ l , n = 27) ( P < 0.0001). High levels of IL‐8 on admission dropped sequentially. Significant correlations were found between IL‐8 levels and either neutrophil counts ( r = 0.612), G‐CSF levels ( r = 0.873) or MIP‐1α levels ( r = 0.623) in the CSF of the affected patients ( P < 0.0001). IL‐8 values in serum were lower than in the corresponding CSF samples from all individuals with meningeal symptoms. The IL‐8 mRNA was detectable by reverse‐transcribed polymerase chain reaction (PCR)‐assisted amplification in fresh leucocytes from the CSF, but not from the peripheral blood of a healthy volunteer. The culture of CSF mononuclear cells produced high levels of IL‐8 (∼ 2750 ng/ l ). These data indicate that IL‐8 levels rise transiently at the initial stage of aseptic meningitis, and that mononuclear cells that migrate into the CSF are a cellular source of this chemokine. We suppose that IL‐8, in addition to G‐CSF and MIP‐1α, contribute to the localized neutrophil accumulation during the disease.