Open AccessComplement coating of erythrocytes is reduced following their interaction with neutrophils in vitro without loss of complement receptor 1 (CR1)
Author(s) -
IMRIE H. J.,
JONES D. R. E.
Publication year - 1997
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1997.4151312.x
Subject(s) - complement (music) , immunology , in vitro , complement receptor , receptor , complement receptor 1 , complement system , complement component 5 , medicine , biology , antibody , genetics , phenotype , complementation , gene
We have investigated the interaction of complement‐coated erythrocytes (E) with neutrophils, in vitro , to determine the effects of erythrocyte CR1. Complement‐coating in vitro , to mimic immune complex uptake, caused a reduction in E‐CR1, but subsequent interaction with neutrophils effected a removal of E‐C3b and a return of E‐CR1 to levels approximating that of uncoated erythrocytes. These data indicate that C3b, associated with immune complexes, may bind to E‐CR1 in a reversible manner and that subsequent interaction with phagocytes need not necessarily result in cleavage (and loss of ) E‐CR1. Further, we conclude that E‐CR1 can apparently be reduced by epitope masking.