Open AccessThe priming action of tumour necrosis factor‐alpha (TNF‐α) and granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) on neutrophils activated by inflammatory microcrystals
Author(s) -
BURT H. M.,
JACKSON J. K.
Publication year - 1997
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1997.4011298.x
Subject(s) - degranulation , tumor necrosis factor alpha , myeloperoxidase , phagocytosis , granulocyte , immunology , granulocyte macrophage colony stimulating factor , lysozyme , zymosan , respiratory burst , inflammation , chemistry , cytokine , microbiology and biotechnology , medicine , biology , biochemistry , in vitro , receptor
We studied the effects of TNF‐α or GM‐CSF on the production of reactive oxygen species (as measured by chemiluminescence) and degranulation responses of neutrophils to opsonized inflammatory microcrystals. TNF‐α in the 10–2000 p m or GM‐CSF in the 2–200 p m concentration range caused the concentration‐dependent amplification of neutrophil chemiluminescence responses to both calcium pyrophosphate dihydrate (CPPD) and monosodium urate monohydrate (MSUM) crystals. Degranulation responses, as measured by the extracellular release of the granule enzymes myeloperoxidase or lysozyme, were amplified by ≈ 50–100% for both MSUM or CPPD crystal‐induced neutrophil activation when cells were pretreated with TNF‐α at 2000 p m or GM‐CSF at 75 p m .