Open AccessNon‐restricted T cell receptor (TCR)‐Vα and ‐Vβ gene usage in patients with pulmonary sarcoidosis
Author(s) -
HOSHINO T.,
SUZUKI R.,
TSURUTA Y.,
MATSUTANI T.,
KIKUCHI M.,
KAWAMOTO M.,
GOUHARA R.,
SHIRAISHI T.,
MOCHIZUKI M.,
ITOH K.,
OIZUMI K.
Publication year - 1997
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1997.3821279.x
Subject(s) - t cell receptor , sarcoidosis , immunology , bronchoalveolar lavage , peripheral blood mononuclear cell , medicine , biology , microbiology and biotechnology , t cell , lung , pathology , immune system , genetics , in vitro
Sarcoidosis is a systemic granulomatous disease of unknown etiology characterized by the pronounced accumulation of CD4 + T cells and macrophages in the affected organs. TCR variable (V)α and Vβ gene usage in patients with sarcoidosis is still a matter of discussion. In this investigation, we analysed TCR‐Vα and ‐Vβ gene usage in bronchoalveolar lavage fluid (BALF) and peripheral blood mononuclear cells (PBMC) of 30 patients with active pulmonary sarcoidosis using an adapter ligation method, reverse transcriptase‐polymerase chain reaction (RT‐PCR), and sequence‐specific oligonucleotide probe (SSOP) analyses. There was no significant difference in TCR‐Vα or ‐Vβ gene usage between BALF ( n =12) or PBMC ( n =27) of patients and PBMC of healthy subjects ( n =10). Neither selective TCR‐Vα nor ‐Vβ expansion was observed in the paired BALF and PBMC from seven of nine patients. However, selective expansions were observed in a few TCR‐Vα or ‐Vβ subsets in the BALF or PBMC of some individuals. Although a modest increase in a few TCR‐Vα or ‐Vβ subsets was observed in the BALF or PBMC of some individuals, the increased TCR‐Vα or ‐Vβ subsets were not closely associated with the HLA‐DRB1, DQA1, DQB1, and DPB1 alleles of these patients. These results suggest that TCR‐Vα or ‐Vβ gene usage is not restricted in both lung and peripheral blood in the majority of patients with active pulmonary sarcoidosis.