Open AccessRespiratory syncytial virus‐induced cytokine production by neonatal macrophages
Author(s) -
TSUTSUMI H.,
MATSUDA K.,
SONE S.,
TAKEUCHI R.,
CHIBA S.
Publication year - 1996
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1996.d01-874.x
Subject(s) - biology , virus , immunology , cytokine , mononegavirales , tumor necrosis factor alpha , immune system , interferon , paramyxoviridae , pneumovirinae , reverse transcriptase , virology , macrophage , secretion , in vitro , polymerase chain reaction , viral disease , gene , endocrinology , biochemistry
The induction of immunoregulatory cytokines IL‐1β, IL‐6, IL‐12, tumour necrosis factor‐alpha (TNF‐α) and interferon‐gamma (IFN‐γ) was studied with neonatal (cord blood) monocyte‐derived macrophages (MDM) after in vitro infection with respiratory syncytial virus (RSV). The expression of mRNAs for these cytokines in RSV‐infected MDM was examined by reverse transcriptase‐polymerase chain reaction (RT‐PCR). The activities of these cytokines were assayed by ELISA. Significant increase of expression of mRNA for IL‐6, IL‐12, TNF‐α and IFN‐γ occurred within 2 h after infection and decreased within 6 h after infection. At 20 h after infection the MDM produced and secreted moderate levels of IL‐6 and TNF‐α; however, no IL‐12 and IFN‐γ activities were detected. Moderate IL‐1β mRNA was expressed before RSV infection, and its expression increased at 2 h after infection. However, no detectable IL‐1β was secreted in culture fluids. These observations suggest that RSV‐infected neonatal macrophages produce and secrete IL‐6 and TNF‐α quickly during the eclipse phase of RSV infection and therefore may play a prominent role in the initiation of the immune response to RSV.