Open AccessHeat shock‐enhanced T cell apoptosis with heat shock protein 70 on T cell surface in multicentric Castleman's disease
Author(s) -
ISHIYAMA T.,
KOIKE M.,
AKIMOTO Y.,
FUKUCHI K.,
WATANABE K.,
YOSHIDA M.,
WAKABAYASHI Y.,
TSURUOKA N.
Publication year - 1996
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1996.d01-829.x
Subject(s) - apoptosis , heat shock protein , peripheral blood mononuclear cell , hsp70 , shock (circulatory) , hyperthermia , heat shock , cell , flow cytometry , programmed cell death , microbiology and biotechnology , biology , immunology , chemistry , medicine , in vitro , biochemistry , gene
We report here that T cells from patients with multicentric Castleman's disease (MCD) are sensitive to hyperthermia. T cells from two of three patients with MCD revealed DNA ladder formation and chromatin condensation following heat shock (30 min at 41.5°C). Peripheral blood mononuclear cells (PBMC) from the same MCD patients exhibited high levels of spontaneous apoptosis after 72 h in culture and elevated apoptosis after heat shock, as evaluated by a quantitative flow cytometric assay. Heat shock protein 70 (hsp70) was detected on the cell surface of T cells in all three patients after heat shock. Furthermore, hsp70 was detected on T cells in the two MCD patients with apoptosis even in the absence of heat shock. T cells from normal samples did not show either heat‐shock‐induced expression of cell‐surface hsp70 or apoptosis. Thus, heat shock treatment augmented hsp70 expression on the cell surface of T cells and enhanced apoptosis. Our studies suggest that hyperthermia may influence the clinical course of MCD.