Open AccessRelease of transforming growth factor‐beta (TGF‐β) and fibronectin by alveolar macrophages in airway diseases
Author(s) -
VIGNOLA A. M.,
CHANEZ P.,
CHIAPPARA G.,
MERENDINO A.,
ZINNANTI E.,
BOUSQUET J.,
BELLIA V.,
BONSIGNORE G.
Publication year - 1996
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1996.d01-811.x
Subject(s) - chronic bronchitis , bronchoalveolar lavage , fibronectin , immunology , medicine , transforming growth factor beta , lipopolysaccharide , alveolar macrophage , pulmonary alveolus , lung , bronchitis , macrophage , transforming growth factor , respiratory disease , chemistry , cell , in vitro , biochemistry
Asthma and chronic bronchitis are associated with airway remodelling, and airway macrophages are present in bronchial inflammation. TGF‐β and fibronectin released by alveolar macrophages possess a fibrogenic potency. The potential role of alveolar macrophages in airway remodelling was studied in asthma and chronic bronchitis by the release of TGF‐β and fibronectin. Alveolar macrophages were isolated by bronchoalveolar lavage in 14 control subjects, 14 asthmatics and 14 chronic bronchitics. The spontaneous and lipopolysaccharide (LPS)‐ or concanavalin A (Con A)‐induced release of TGF‐β and fibronectin was measured by ELISA. Alveolar macrophages from chronic bronchitics spontaneously release greater amounts of TGF‐β and fibronectin than those from asthmatic and control subjects. Alveolar macrophages from asthmatics release greater amounts of TGF‐β and fibronectin than those from control subjects. The spontaneous release of TGF‐β is significantly correlated with that of fibronectin. Fibronectin release was significantly reduced after LPS stimulation, and TGF‐β release was significantly increased after LPS stimulation, except in chronic bronchitis patients. Con A increased the release of TGF‐β in cells from normal subjects. This study suggests that activated macrophages play a role in airway remodelling in chronic bronchitis and to a lesser extent in asthma.