Open AccessMeasurement of anticardiolipin antibodies by ELISA using β 2 ‐glycoprotein I (β 2 ‐GPI) in systemic sclerosis
Author(s) -
IHN H.,
SATO S.,
FUJIMOTO M.,
KIKUCHI K.,
IGARASHI A.,
SOMA Y.,
TAMAKI K.,
TAKEHARA K.
Publication year - 1996
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1996.d01-774.x
Subject(s) - isotype , medicine , immunology , serology , anticardiolipin antibodies , antibody , connective tissue disease , immunopathology , systemic disease , autoantibody , clinical significance , lupus erythematosus , autoimmune disease , monoclonal antibody
In order to determine the prevalence and clinical significance of β 2 ‐GPI‐dependent anticardiolipin antibodies (β 2 ‐GPI/aCL) in patients with systemic sclerosis (SSc), serum samples from 80 patients with SSc, 20 patients with systemic lupus erythematosus (SLE), and 120 healthy control subjects were examined by ELISA using purified β 2 ‐GPI. IgG isotype β 2 ‐GPI/aCL was present in eight of 80 patients with SSc (10%), and the presence of β 2 ‐GPI/aCL IgG was significantly correlated with the presence of isolated pulmonary hypertension (PH). Furthermore, levels of β 2 ‐GPI/aCL IgG were significantly correlated with levels of mean pulmonary arterial pressure. These data suggest that IgG isotype β 2 ‐GPI/aCL might be a serological indicator of the severity of PH in patients with SSc.