Open AccessAnti‐neutrophil cytoplasmic antibodies (ANCA) in ulcerative colitis: anti‐cathepsin G and a novel antibody correlate with a refractory type
Author(s) -
SOBAJIMA J,
OZAKI S,
OKAZAKI T,
OSAKADA F,
SUMITA S,
MORI K,
NAKAO K
Publication year - 1996
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1996.d01-724.x
Subject(s) - ulcerative colitis , immunology , antibody , anti neutrophil cytoplasmic antibody , cathepsin g , autoantibody , medicine , refractory (planetary science) , colitis , biology , vasculitis , pathology , disease , proteases , biochemistry , astrobiology , enzyme
We analysed the clinical significance of ANCA in patients with ulcerative colitis. On either an indirect immunofluorescence assay or an ELISA with fixed neutrophils, 71% (25/35) of the patients were positive for ANCA. However, only half of them reacted with either cathepsin G or lactoferrin. Western blot assays revealed positive bands in 40% (10/25) of the antibody‐positive patients. The sizes of the bands detected were ≈58, 47, 44, 40, and 28 kD. No significant correlation was found between the ANCA positivity and various variables, i.e. disease activity, extent of lesion, or treatment of the disease. The anti‐cathepsin G and 28‐kD positivity, however, significantly correlated with a refractory type of ulcerative colitis.