Open AccessAnti‐DNA antibody derived from a systemic lupus erythematosus (SLE) patient forms histone–DNA–anti‐DNA complexes that bind to rat glomeruli in vivo
Author(s) -
MORIOKA T.,
FUJIGAKI Y.,
BATSFORD S. R.,
WOITAS R.,
OITE T.,
SHIMIZU F.,
VOGT A.
Publication year - 1996
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1996.d01-658.x
Subject(s) - dna , histone , immunology , in vivo , lupus erythematosus , antibody , systemic lupus erythematosus , autoantibody , biology , medicine , genetics , pathology , disease
Histone can mediate the binding of both free DNA and DNA complexed to anti‐DNA antibody to the glomerular capillary wall. We tested whether preformed histone–DNA–anti‐DNA immune complexes (IC) could bind to the glomerular capillary wall. The immune complex, generated with anti‐DNA antibody derived from an SLE patient and excess of 125 I‐DNA followed by digestion with DNase, was mixed with histones. The complex containing 4 μg DNA was injected via the aorta into the left kidney of rats. At 15 min, 1.3% of the histone–DNA–anti‐DNA antibody complex bound (measured as 125 I‐DNA), when histone was omitted less than 0.1% of the DNA–anti‐DNA antibody complex bound. By immunofluorescence human immunoglobulins and histones, representing the IC, could be observed in a capillary pattern; but no complement deposition was detected. Electron microscopy revealed discrete, electron dense deposits in a subendothelial, subepithelial and mesangial localization at 15 min. These results provide direct evidence that antibodies from serum of SLE patients can form soluble histone–DNA–anti‐DNA immune complexes that bind to the glomerular capillary wall in vivo