Open Accessγδ T lymphocyte responses to HIV
Author(s) -
WALLACE M.,
BARTZ S. R.,
CHANG W.L.,
MACKENZIE D. A.,
PAUZA C. D.,
MALKOVSKY M.
Publication year - 1996
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1996.d01-625.x
Subject(s) - clone (java method) , gamma delta t cell , cytotoxic t cell , t lymphocyte , virology , biology , t cell , immunology , cellular immunity , lytic cycle , t cell receptor , virus , immune system , in vitro , genetics , gene
Natural immunity may be involved in controlling viral spread in hosts infected with HIV. A panel of γδ T cell receptor‐positive lymphocyte clones was isolated from the peripheral blood of healthy HIV − donors and tested for anti‐HIV cytotoxic responses. Twelve of 30 (40%) Vγ9 + Vδ2 + T cell clones, but none of seven Vδ1 + T cell clones, displayed lytic activity against HIV‐infected cells. The Vγ9 + /Vδ2 + clones cytotoxic for HIV‐infected cells also lysed Daudi cells. However, not all Vγ9 + /Vδ2 + clones which lysed Daudi targets had the capacity to lyse HIV‐infected cells. Some of the γδ T cell clones were also investigated for potential proliferative responses to HIV‐infected cells. One Vγ9 + /Vδ2 + T cell clone (ME8‐7) and one Vδ1 + T cell clone (ME18‐2) demonstrated proliferative responses toward HIV‐infected cells. Another Vγ9 + /Vδ2 + clone (VM39) proliferated in response to cell‐free HIV. Taken together, these results provide direct evidence of anti‐HIV γδ T cell responses in healthy, HIV − persons.