Open AccessSuccessful induction of severe destructive arthritis by the transfer of in vitro ‐activated synovial fluid T cells from patients with rheumatoid arthritis (RA) in severe combined immunodeficient (SCID) mice
Author(s) -
SAKATA A.,
SAKATA K.,
PING H.,
OHMURA T.,
TSUKANO M.,
KAKIMOTO K.
Publication year - 1996
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1996.979670.x
Subject(s) - synovial fluid , immunology , medicine , arthritis , rheumatoid arthritis , pathogenesis , in vitro , adoptive cell transfer , peritoneal cavity , immune system , pathology , t cell , biology , surgery , biochemistry , alternative medicine , osteoarthritis
In order to investigate the role of pathogenic T cells in RA, the establishment of an RA model using patients’ T cells is thought to be essential. In this study, multiple and severe destructive arthritis was established by transferring in vitro ‐stimulated synovial fluid T (SFT) cells from patients with RA through simultaneous injection into knee joint and peritoneal cavity of SCID mice without causing xenogeneic graft‐ versus‐ host disease (GVHD). Neither the transfer of unstimulated SFT cells nor sole i.p. injection was sufficient to induce severe arthritis. Interestingly, in contrast with SFT cells, in vitro ‐activated peripheral blood lymphocytes from RA patients failed to trigger such arthritis, suggesting that pathogenic T cells might be concentrated in synovial fluid of RA patients. This, the first severe arthritis model mimicking RA induced by RA patients’ T cells, is expected to provide important information about RA pathogenesis and a possible therapeutic approach.