Open AccessMitogenic effect of HIV‐infected human T cell lines on mouse B cells mediated by surface immunoglobulin
Author(s) -
ARASE N.,
ARASE H.,
OHKI K.,
NISHINO Y.,
IKUTA K.,
ONOÉ K.
Publication year - 1996
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1046/j.1365-2249.1996.901593.x
Subject(s) - biology , cell culture , antibody , virology , t cell , b cell , immunology , microbiology and biotechnology , immune system , genetics
Following HIV‐1 infection, a number of disorders are induced in both normal T and B cells by virus products derived from infected CD4 + T cells. In the present study, we found that HIV‐infected, but not uninfected, human T cell lines generated vigorous blastogenesis and proliferation of freshly isolated mouse B cells in a short‐term culture. Neither human B cells nor rat B cells showed significant responses to the HIV‐infected T cell lines in the present condition. The mitogenic effect of HIV‐infected human T cell line requires direct cell–cell interaction between mouse B cells and HIV‐infected T cell lines. Since either mitomycin c treatment or paraformaldehyde fixation of HIV‐infected T cell lines resulted in complete loss of the mitogenic effect, it seems that de novo synthesized viral products are responsible for this effect. Furthermore, anti‐mouse immunoglobulin antibody inhibited completely the B cell stimulation by the HIV‐infected human T cell lines. Thus, surface immunoglobulin (sIg) on mouse B cells appears to be an essential molecule which transduces activation signals from HIV‐infected human T cells into cytoplasm of the B cells.