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Expression of CS‐1 fibronectin precedes monocyte chemoattractant protein‐1 production during elicitation of allergic contact dermatitis
Author(s) -
Martín A. P.,
Gagliardi J.,
BaenaCagnani C. E.,
Eberhard Y.,
Uguccioni M.,
Gallino N.,
Mariani A. L.,
Serra H. M.
Publication year - 2003
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1046/j.1365-2222.2003.01712.x
Subject(s) - ccl17 , chemokine , monocyte , fibronectin , immunology , allergic contact dermatitis , peripheral blood mononuclear cell , ccl2 , chemistry , cxcr3 , biology , inflammation , microbiology and biotechnology , cxcl10 , allergy , chemokine receptor , extracellular matrix , in vitro , biochemistry
Summary Background Leucocyte migration within inflammatory skin compartments in allergic contact dermatitis (ACD) is the result of a sophisticated multi‐step event where multiple molecules are involved. Objective Since non‐antigen‐specific mechanisms have been described as an early participant in elicitation of ACD, we investigated the kinetics of the expression of monocyte chemoattractant protein‐1 (MCP‐1/CCL2) and the type of infiltrating cells. We compared the time course production of MCP‐1/CCL2 with connecting segment‐1 (CS‐1) fibronectin and thymus and activation‐regulated chemokine (TARC/ CCL17) expression. Methods Biopsies from 10 individuals challenged in their back with the antigen responsible for their contact dermatitis and an irrelevant antigen were taken at different times and histology, immunohistochemistry for CS‐1 fibronectin, TARC/CCL17, CD3, CD68, CXCR3, CCR4 and in situ hybridization for MCP‐1/CCL2 were performed. Results At positive antigen stimulated sites expression of MCP‐1/CCL2 by basal keratinocytes and isolated cells in dermis started at 10 h. CS‐1 fibronectin and TARC/CCL17 expression by blood endothelial cells was found at 2 and 10 h, respectively. This was followed by dermal accumulation of mononuclear cells with a significant increase of CD3 + and CD68 + cells. At 48 h, approximately 58% of infiltrating cells were CXCR3 + , and 35% CCR4 + . Conclusions We showed evidence of the fact that CS‐1 fibronectin expression precedes the production of MCP‐1/CCL2 and TARC/CCL17 in the skin of patients with ACD, suggesting that these molecules participate in the early complex process of migrating mononuclear cells during elicitation of ACD.