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Assessment of oxidant stress in allergic asthma by measurement of the major urinary metabolite of F 2 ‐isoprostane, 15‐ F 2t ‐IsoP (8‐iso‐PG F 2α )
Author(s) -
Dworski R.,
Jackson Roberts Ii L.,
Murray J. J.,
Morrow J. D.,
Hartert T. V.,
Sheller J. R.
Publication year - 2001
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1046/j.1365-2222.2001.01055.x
Subject(s) - asthma , excretion , methacholine , metabolite , inhalation , urinary system , urine , oxidative stress , provocation test , isoprostane , medicine , allergen , in vivo , chemistry , exhaled breath condensate , immunology , pharmacology , endocrinology , allergy , pathology , anesthesia , lipid peroxidation , lung , respiratory disease , biology , alternative medicine , microbiology and biotechnology
Asthma is a chronic inflammatory disease of the airways which may involve an oxidant injury to the lung. Assessment of oxidant stress is difficult in vivo , but measurement of F 2 ‐isoprostanes ( F 2 ‐IsoPs), free radical‐catalysed products of arachidonic acid, appears to offer a reliable approach for quantitative measurement of oxidative stress status in vivo . We have recently developed a mass spectrometric assay for 2,3‐dinor‐5,6‐dihydro‐15‐ F 2t ‐IsoP (15‐ F 2t ‐IsoP‐M), the major urinary metabolite of the F 2 ‐IsoP, 15‐ F 2t ‐IsoP (8‐iso‐PG F 2a ). Measurement of the urinary excretion of this metabolite offers a reliable index of oxidative stress status in vivo that has advantages over measuring unmetabolized F 2 ‐IsoPs in urine and plasma. To assess the occurrence of oxidative stress in patients with atopic asthma following allergen exposure in vivo by measuring the urinary excretion of 15‐ F 2t ‐IsoP‐M. Analysis of 15‐ F 2t ‐IsoP‐M by GC‐NICI‐MS in nine mild atopic asthmatics following inhaled allergen provocation and four asthmatic subjects after inhaled challenge with methacholine. Urinary excretion of 15‐ F 2t ‐IsoP‐M increased at 2 h after allergen challenge and remained significantly elevated in all urine collections during the subsequent 8‐h period of the study compared to the baseline value ( anova , and Student–Newman–Keuls multiple comparisons test). No increase in the urinary excretion of 15‐ F 2t ‐IsoP‐M occurred after inhalation of methacholine. Allergen challenge causes an oxidant injury in human atopic asthmatics. 15‐ F 2t ‐IsoP‐M is a valuable marker of oxidant stress in vivo .