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Altered in vitro apoptosis of cultured mast cells prepared from an inbred strain of mice, NC/Kuj
Author(s) -
Takaaki Hiragun,
Eishin Morita,
Hajime Shindo,
Toshihiko Tanaka,
Yoshikazu Kameyoshi,
Tsutomu Okabe,
Masamoto Kanno,
Shoso Yamamoto
Publication year - 2000
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1046/j.1365-2222.2000.00726.x
Subject(s) - histamine , apoptosis , mast cell , in vitro , immunoglobulin e , hyperplasia , bone marrow , microbiology and biotechnology , chemistry , immunology , biology , endocrinology , antibody , biochemistry
Background An inbred strain of mice, NC, develops dermatitis associated with highly elevated serum IgE and dermal mast cell hyperplasia. Objectives and methods To clarify the mechanisms for the dermal mast cell hyperplasia in NC, we prepared bone marrow‐derived mast cells (BMMCs) from three strains of mice, NC/Kuj, C57BL/6 and BALB/c, and compare histamine contents, histamine release abilities, adhesive properties and apoptosis of the BMMCs. Results Compared with BMMCs obtained from C57BL/6 and BALB/c, NC/Kuj BMMC possessed higher histamine content and higher adhesive ability to plastic plates, although histamine release from BMMCs was found to be similar in the three strains. The most intriguing finding is the lack of apoptosis in the BMMCs from NC/Kuj upon growth factor deprivation as determined by DNA ladder formation and loss of membrane phospholipid asymmetry. Conclusion The altered in vitro properties of mast cells in NC/Kuj partially account for an increase of dermal mast cells, which might be involved in the development of skin lesions in NC.