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Bronchoprotective effects of atrial natriuretic peptide against propranolol‐induced bronchoconstriction after allergic reaction in guinea pigs
Author(s) -
Masayuki Mizuguchi,
Saori Myo,
Masaki Fujimura
Publication year - 2000
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1046/j.1365-2222.2000.00725.x
Subject(s) - bronchoconstriction , propranolol , ovalbumin , histamine , atrial natriuretic peptide , guinea pig , medicine , inhalation , pharmacology , endocrinology , immunology , antigen , anesthesia , asthma
Objective To study the effects of intravenous atrial natriuretic peptide (ANP) on antigen‐induced bronchoconstriction, propranolol‐induced bronchoconstriction (PIB) after antigen challenge, and histamine‐induced bronchoconstriction in guinea pigs. Methods Allergic bronchoconstriction was evoked by inhalation of ovalbumin (OA) and PIB was caused when 10 mg/mL of propranolol was inhaled 20 min after OA challenge in passively sensitized and artificially ventilated guinea pigs. 25, 50, 100 and 200 μg/mL of histamine were inhaled for 20 s at 5‐min intervals in non‐sensitized guinea pigs. Results Pretreatment with ANP in doses of 0.1 and 1.0 nmol/kginjected intravenously 15 min after antigen challenge reduced PIB in a dose‐dependent manner, and 5 min before antigen challenge significantly attenuated PIB but not antigen‐induced bronchoconstriction. Intravenous ANP significantly reduced bronchial responses to increasing concentrations of inhaled histamine in a dose‐dependent manner. Conclusion These results suggest that ANP possesses protective effects against propranolol‐induced and histamine‐induced bronchoconstriction, albeit by a non‐specific mechanism in guinea pig in vivo .