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Adenoviral infection inhibits allergic airways inflammation in mice
Author(s) -
Martin R. Stämpfli,
Stacey A. Ritz,
G. Scott Neigh,
Patricia J. Sime,
X F Lei,
Zhou Xing,
Kenneth Croitoru,
Manel Jordana
Publication year - 1998
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1046/j.1365-2222.1998.00446.x
Subject(s) - immunology , ovalbumin , bronchoalveolar lavage , cytokine , immune system , medicine , inflammation , t cell , antigen , immunoglobulin e , lung , antibody
Background Recent epidemiological studies have suggested that exposure to certain viruses and bacteria influences the development of allergy and allergic diseases, such as asthma. However, there is a paucity of experimental evidence examining the consequences of concurrent exposure to allergen and infectious agents, and the potential mechanisms by which allergic disease might be averted as a result. Objective To model this situation experimentally, we investigated whether a virally induced immune response, elicited by a replication‐deficient human type 5 adenovirus (RDA) administered at a site distant from the airways, could inhibit ovalbumin (OVA)‐induced airways eosinophilic inflammation. Methods C57BL/6 mice were infected intramuscularly with RDA 16 h prior to intraperitoneal OVA sensitization. Cellular and cytokine responses in the lung/airways were examined after an OVA aerosol challenge. Results RDA infection significantly inhibited the inflammatory response in the lung tissue after antigen challenge. In the bronchoalveolar lavage (BAL), total cell number, eosinophils and lymphocytes were decreased by 70, 85 and 65%, respectively, after antigen challenge in RDA‐treated, compared with untreated, mice. RDA infection had no effect on IgE synthesis. The levels of IL‐5, IL‐4 and IFNγ in the BAL after antigen challenge were significantly lower in RDA‐treated mice. In vitro production of cytokines by splenocytes in response to OVA restimulation revealed a shift from IL‐4 in sensitized, PBS‐treated mice, to IFNγ in sensitized mice treated with RDA. Flow cytometric analysis revealed that RDA infection increased the proportion of CD8 T cells in the BAL; this change in T‐cell subsets was accompanied by an increase in both CD4 and CD8 T cells positive for intracellular IFNγ. Inhibition of antigen‐induced airways inflammation was IFNγ‐dependent but did not require IL‐12, as RDA‐treatment inhibited airways inflammation in IL‐12 but not IFNγ knock‐out mice. Conclusion This study demonstrates that an immune response against a replication‐deficient adenovirus during the initial exposure to OVA inhibits the development of airways inflammation after antigen aerosol challenge.