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Circulating CLA + lymphocytes from children with atopic dermatitis contain an increased percentage of cells bearing staphylococcal‐related T‐cell receptor variable segments
Author(s) -
Mauricio Torres,
Francisco García González,
José Luís Corzo,
María D. Girón,
M.J. Carvajal,
Verónica Edith García,
A. Bodas Pinedo,
Antonio MartínezValverde,
Miguel Blanca,
L.F. Santamaría
Publication year - 1998
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1046/j.1365-2222.1998.00397.x
Subject(s) - atopic dermatitis , superantigen , immunology , t cell receptor , medicine , staphylococcus aureus , t cell , t lymphocyte , allergy , antigen , cd3 , immune system , biology , cd8 , bacteria , genetics
Background Atopic dermatitis is an allergic T‐cell mediated skin inflammation. Staphylococcus aureus colonization is very common in cutaneous atopic dermatitis lesions. The cutaneous lymphocyte‐associated antigen (CLA) is a T cell skin homing receptor that defines T lymphocytes associated with the cutaneous immune response. Objective To study whether CLA + T cells from atopic dermatitis children present a selective expression for Staphylococcus aureus ‐related TCR Vβ segments. Methods Peripheral blood T cells were stained with HECA‐452 (anti‐CLA) and a panel of TCR Vβ specific monoclonal antibodies and analysed by flow cytometry. Results Atopic dermatitis patients have a higher percentage of circulating CLA + CD3 + lymphocytes compared with healthy controls. Patients with active atopic dermatitis during the study expressed a higher percentage of cells positive for the TCR Vβ2 and Vβ5.1 segments in the CLA + but not in the CLA − subset. These TCR Vβs are recognized by staphylococcal superantigens. Moreover, there was an increased percentage of HLA‐DR + expression by CLA + Vβ5.1 + T cells in patients with active atopic dermatitis, but those patients whose eczema was inactive had very similar values to healthy controls regarding TCR Vβ and HLA‐DR phenotype in circulating CLA + T lymphocytes. Conclusion Our data indicate that circulating skin‐homing T cells of patients with active atopic dermatitis contain an increased percentage of cells bearing TCR Vβ segments related with Staphylococcus aureus . Staphylococcus superantigens may therefore trigger expansion or at least circulation of appropriate CLA + T cells.