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Allergen challenge‐induced extravasation of plasma in mouse airways
Author(s) -
Jonas Erjefält,
Paul Andersson,
Bàrbro Gustafsson,
Magnus Korsgren,
Birgitta Sonmark,
C. G. A. Persson
Publication year - 1998
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1046/j.1365-2222.1998.00372.x
Subject(s) - extravasation , pathology , medicine , immunology , albumin , vascular permeability , airway , blood proteins , endocrinology , anesthesia
Background Mouse models are extensively used to study genetic and immunological mechanisms of potential importance to inflammatory airway diseases, e.g. asthma. However, the airway pathophysiology in allergic mice has received less attention. For example, plasma extravasation and the ensuing tissue‐deposition of plasma proteins, which is a hallmark of inflammation, has not been examined in allergic mice. Objective This study aims to examine the vascular permeability and the distribution of plasma proteins in mouse airways following exposure to allergen and serotonin. Methods Extravasated plasma was quantified by a dual isotop technique using intravascular ( 131 I‐albumin) and extrasvascular ( 125 I‐albumin) plasma tracers. Histological visualization of fibrinogen and colloidal gold revealed the tissue distribution of extravasated plasma. Results Allergen aerosol exposure (3% OVA, 15min) of sensitized animals resulted in a marked plasma extravasation response in the trachea ( P < 0.01) and the bronchi but not in the lung parenchyma. A similar extravasation response was induced by serotonin ( P < 0.001). Extravasating vessels (assessed by Monastral blue dye) were identified as intercartilaginous venules. Extravasated plasma abounded in the subepithelial tissue but was absent in the epithelium and airway lumen. The allergen‐induced response was dose‐dependently inhibited by iv administration of formoterol ( P < 0.001), a vascular antipermeability agent. Conclusion The present study demonstrates that serotonin and allergen challenge of sensitized mice increase airway venular permeability to cause transient extravasation and lamina propria distribution of plasma in the large airways. We suggest that the extravasation response is a useful measure of the intensity and the distribution of active inflammation