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GC/MS analysis of urinary excretion of 9α,11β‐PGF 2 in acute and exercise‐induced asthma in children
Author(s) -
Nagakura T.,
Obata T.,
Shichijo K.,
Matsuda S.,
Sigimoto H.,
Yamashita K.,
Masaki T.,
Maekawa K.
Publication year - 1998
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1046/j.1365-2222.1998.00187.x
Subject(s) - asthma , urinary system , urine , medicine , metabolite , excretion , prostaglandin f , endocrinology , prostaglandin
Background 9α,11β‐Prostaglandin (PG) F 2 is an initial metabolite of PGD 2 which has a potent bronchoconstrictive activity. Objectives We measured the urinary levels of 9α,11β‐PGF 2 in asthmatic children to investigate its role in not only acute asthmatic attack in a time course study but also in exercise‐induced asthma (EIA). Methods In the acute asthma study, 30 asthmatic children were examined. Urine samples were collected on the first, third, and sixth days. Urinary levels of 9α,11β‐PGF 2 were measured with gas chromatography mass spectrometry using the electron impact method. In the exercise challenge study, 14 children with EIA and 14 children without EIA were studied. Urine samples were collected before exercise challenge, and at 1 h, and 5 h after exercise challenge. Urinary levels of 9α,11β‐PGF 2 were measured. Results Elevated urinary levels of 9α,11β‐PGF 2 , which were observed on the first day when treatment was started in the hospital, were gradually decreased on the third day ( P < 0.05), and on the sixth day ( P < 0.01). A significant correlation between urinary levels of 9α,11β‐PGF 2 and symptom scores ( P < 0.005) was observed on the first day. In EIA, there was a significant increase in urinary levels of 9α,11β‐PGF 2 at 1 h ( P < 0.01) and at 5 h ( P < 0.01) after exercise challenge, but not in the children without EIA. Conclusion 9α,11β‐PGF 2 may be involved in the pathogenesis of acute and exercise‐induced asthma in children.