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A method for canine nasal mucosa superfusion, in vivo; evidence that pseudomonas‐induced neutrophil migration is inhibited by α 2 ‐adrenoceptor agonist
Author(s) -
STJÄRNE P.,
AGUSTI C.,
CARDELL L. O.,
WAGSTAFF S.,
NADEL J. A.
Publication year - 1997
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1046/j.1365-2222.1997.1740967.x
Subject(s) - oxymetazoline , agonist , mucous membrane of nose , in vivo , neutrophile , exotoxin , pharmacology , chemistry , endocrinology , biology , medicine , immunology , receptor , microbiology and biotechnology , inflammation , toxin
Summary Methods and Results A method for isolating and superfusing a segment of the nasal cavity in the anaesthetized dog was developed. Introduction of Pseudomonas aeruginosa supernatant (PA), which is known to induce IL‐8 release, resulted in a marked time‐dependent exponential increase in neutrophil recruitment to the nasal chamber. When oxymetazoline (final concentration. 10 ‐5 M), was added to the superfusate, the PA induced‐neutrophil migration was inhibited; the addition of benzalchoniul chloride to the superfusate had no effect on the PA induced‐neutrophil migration. Conclusion We conclude that a α 2 adrenoceptor agonist profoundly inhibits neutrophil migration in response to bacterial products. Our novel method allows continuous introduction of stimuli and monitoring of responses in the nasal mucosa.