Open AccessOctaploid Meth‐A cells are established from a highly polyploidized cell population
Author(s) -
FujikawaYamamoto Kohzaburo,
Yamagishi Hiroko,
Miyagoshi Minoru
Publication year - 2003
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1046/j.1365-2184.2003.00260.x
Subject(s) - meth , ploidy , biology , cell , population , microbiology and biotechnology , genetics , chemistry , gene , medicine , monomer , organic chemistry , environmental health , acrylate , polymer
. Tetraploid Meth‐A cells were polyploidized by demecolcin, an inhibitor of spindle fibre formation in M phase, and then released from the drug 1, 2, 3 and 4 days after the addition. Octaploid cells were successfully established from cell populations including hexadecaploid cells produced by 2, 3 and 4 days of exposure to demecolcin. One‐day‐treated cells were polyploidized octaploid cells, but they returned to tetraploid cells. All of the octaploid Meth‐A cells showed essentially the same features. The octaploid Meth‐A cells had eight homologous chromosomes and double the DNA content of the parent tetraploid cells. The doubling time of octaploid Meth‐A cells was 30.2 h, somewhat longer than the 28.3 and 24.0 h of tetraploid and diploid cells, respectively. The fractions of cells in the G 1 , S and G 2 /M phases were essentially the same in diploid, tetraploid and octaploid Meth‐A cells. The cell volume of octaploid Meth‐A cells was about two times that of the tetraploid cells. It was concluded that octaploid Meth‐A cells were established from transient hexadecaploid cells produced by the polyploidization of tetraploid cells that had been established from diploid cells.