Open AccessEstablishment of a triploid V79 cell line from tetraploid cells obtained through polyploidization using K‐252a
Author(s) -
FujikawaYamamoto Kohzaburo,
Yamagishi Hiroko,
Miyagoshi Minoru
Publication year - 2002
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1046/j.1365-2184.2002.00250.x
Subject(s) - ploidy , biology , karyotype , cell culture , genetics , population , chromosome , microbiology and biotechnology , cell , wi 38 , gene , demography , sociology
. Triploid V79 cells were established from tetraploid cells. Diploid V79 cells were polyploidized by K‐252a, an inhibitor of protein kinases, and then released from the drug for 10 days. At that time, the cell population was a mixture of diploid and tetraploid cells. Triploid cells were obtained through the cloning of tetraploid cells. They had 33 chromosomes (1.5 times the diploid number) and showed a karyotype of three homologueous chromosomes. The duration of the G 1 , S and G 2 /M phases was almost the same as for diploid cells. The cell volume of triploid V79 cells was about two times that of the diploid cells. An explanation for the diploid‐tetraploid‐triploid transition is proposed.