Open AccessSERCA activity is required for timely progression through G1/S
Author(s) -
Simon V. R.,
Moran M. F.
Publication year - 2001
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1046/j.1365-2184.2001.00192.x
Subject(s) - serca , thapsigargin , microbiology and biotechnology , endoplasmic reticulum , intracellular , cytosol , cyclin , mitosis , cell cycle , biology , cyclin dependent kinase , chemistry , atpase , cell , biochemistry , enzyme
. Changes in intracellular Ca 2+ correlate with specific events in the cell cycle. Here we investigated the role of Ca 2+ in the G1 phase. HEK 293 cells were arrested in mitosis and subjected to short‐term treatments that alter Ca 2+ homeostasis prior to their release into G1. Treatment with thapsigargin (TG), an irreversible inhibitor of the sarco‐endoplasmic reticulum Ca 2+ ATPase (SERCA) lengthened the G1 phase. Moreover, TG treatment also resulted in a dramatic alteration in cellular morphology and attachment and in the reduction of MAPK activity and lower levels of cyclin D1 and cyclin E proteins. Treatments with reagents that transiently increase or decrease cytosolic Ca 2+ or that temporarily inactivate SERCA did not alter any of the above parameters. Cells expressing a TG‐resistant form of SERCA progressed normally through the G1/S transition after TG treatment. These results suggest that long‐term SERCA inactivation affects cell cycle‐dependent events and compromises progression through G1/S.