Open AccessMammalian cells are not synchronized in G 1 ‐phase by starvation or inhibition: considerations of the fundamental concept of G 1 ‐phase synchronization
Author(s) -
Cooper S.
Publication year - 1998
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1046/j.1365-2184.1998.00110.x
Subject(s) - synchronization (alternating current) , microbiology and biotechnology , starvation , cell division , mitosis , phase (matter) , biology , cell cycle , dna synthesis , restriction point , dna , cell , chemistry , genetics , cell cycle progression , topology (electrical circuits) , mathematics , combinatorics , organic chemistry , endocrinology
Synchronization of mammalian cells by starvation‐refeeding or by inhibition‐release are among the most commonly used techniques for division cycle analysis. An alternative analysis—in the form of a Gedanken or thought experiment—is presented, casting doubt on the utility of this synchronization method. Arresting cell growth produces a culture where all cells contain a G 1 amount of DNA. However, these cells are not arrested at a particular point in the G 1 ‐phase. Analysis of ‘G 1 arrested cells’ suggests that, upon resumption of growth, the cells are not synchronized.