Open AccessCyclin B1 expression in response to abrogation of the radiation‐induced G 2 /M block in HeLa cells
Author(s) -
Theron T.,
Böhm L.
Publication year - 1998
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1046/j.1365-2184.1998.00107.x
Subject(s) - cyclin b1 , cyclin a , cyclin d , cyclin b , cyclin , microbiology and biotechnology , cell cycle , hela , cyclin a2 , dna damage , biology , chemistry , dna , biochemistry , cell , cyclin dependent kinase 1
The G 2 block is a major response of cells to DNA damage and seem to be induced independently of p53 status. It is thought that the G 2 block has a protective function and allows cells to repair their DNA. The molecular events involved in the formation of the G 2 block therefore are of great interest. We have used pentoxifylline, a potent G 2 delay abrogator, to study the expression of an essential component of the mitosis promoting complex (MPF), cyclin B1. Cyclin B1/G 2 ratios are used to show that irradiation induces a decrease in cyclin B1 expression and that pentoxifylline restores cyclin B1 expression to control level. This confirms that suppression of cyclin B1 plays a role in the formation of the G 2 cell cycle delay, and that elevating cyclin B1 expression is part of the mechanism of action of pentoxifylline on G 2 blocked cells.