Open AccessDirect comparison of bromodeoxyuridine and Ki‐67 labelling indices in human tumours
Author(s) -
Wilson G. D.,
Saunders M. I.,
Dische S.,
Daley F. M.,
Robinson B. M.,
Martindale C. A.,
Joiner B.,
Richman P. I.
Publication year - 1996
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1046/j.1365-2184.1996.00992.x
Subject(s) - bromodeoxyuridine , flow cytometry , immunohistochemistry , labelling , biology , in vivo , cell growth , microbiology and biotechnology , pathology , chemistry , immunology , biochemistry , medicine , genetics
Direct comparison of bromodeoxyuridine (BrdUrd) and Ki‐67 labelling indices was achieved by selecting similar areas from serial sections of human tumours. Fifteen patients were selected who had been administered BrdUrd in vivo and both proliferation markers were assessed by immunohistochemistry. The data show a good correlation between both BrdUrd LI and MIB‐1 LI and T pot (calculated using the flow cytometry derived duration of S phase) and MIB‐1 LI. The contribution of BrdUrd LI to growth fraction varied as a function of proliferation characteristics. In tumours with a high LI, the number of DNA synthesizing cells represented half the growth fraction, whilst in tumours with lower LI's (<10%) the ratio of DNA precursor labelled cells as a function of growth fraction fell to between 10% and 20%. T pot showed a linear correlation with MIB‐1/BrdUrd ratio with a slope approaching unity. It was apparent that both intra‐ and interpatient variation in proliferation index was greater for BrdUrd labelling than for MIB‐1 expression.