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Pure red cell aplasia associated with expansion of CD3 + CD8 + granular lymphocytes expressing cytotoxicity against HLA‐E + cells
Author(s) -
Mori Kiyoshi L.,
Furukawa Hisae,
Hayashi Keiko,
Sugimoto Keiji J.,
Oshimi Kazuo
Publication year - 2003
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2003.04553.x
Subject(s) - cytotoxic t cell , antigen , cytotoxicity , cd3 , human leukocyte antigen , monoclonal antibody , cd8 , t cell , biology , immunology , microbiology and biotechnology , immune system , antibody , biochemistry , in vitro
Summary. T‐cell granular lymphocyte‐proliferative disorder (T‐GLPD) is characterized by the proliferation of cytotoxic T lymphocytes, and is often associated with pure red cell aplasia (PRCA). The mechanism involved in the development of PRCA in T‐GLPD is unknown. Peripheral blood mononuclear cells were isolated from 20 patients with T‐GLPD. Ten patients had associated PRCA. Granular lymphocytes (GLs) of T‐GLPD are positive for CD94, but not NKG2A. To clarify the functional role of CD94 in T‐GLPD, we performed a cytotoxicity assay against the trophoblast cell line, BeWo, which is known to express human leucocyte antigen (HLA)‐E, a natural ligand of CD94, and is deficient in other HLA class I and class II antigens. GLs isolated from T‐GLPD with PRCA patients killed BeWo cells more significantly than GLs from T‐GLPD without PRCA patients. Furthermore, GLs from T‐GLPD with PRCA were significantly stimulated by a monoclonal antibody against CD94, whereas those of T‐GLPD without PRCA were not. Taken together, HLA‐E, a ligand of CD94, was suggested to stimulate CD94 + cells to kill HLA‐E + cells in T‐GLPD with PRCA. GLs of T‐GLPD with PRCA have a potential positive activity against HLA‐E + cells, whereas GLs from T‐GLPD without PRCA do not. CD94 may play a key role in the pathogenesis of PRCA in T‐GLPD.