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Mobilized peripheral blood SSC lo ALDH br cells have the phenotypic and functional properties of primitive haematopoietic cells and their number correlates with engraftment following autologous transplantation
Author(s) -
Fallon Paul,
Gentry Tracy,
Balber Andrew E.,
Boulware David,
Janssen William E.,
Smilee Renee,
Storms Robert W.,
Smith Clay
Publication year - 2003
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2003.04357.x
Subject(s) - haematopoiesis , population , transplantation , cd34 , biology , leukapheresis , stem cell , progenitor cell , immunology , microbiology and biotechnology , side population , andrology , cancer research , cancer stem cell , medicine , environmental health
Summary. We have developed an approach for identifying primitive mobilized peripheral blood cells (PBSC) that express high levels of aldehyde dehydrogenase (ALDH). PBSC were stained with a fluorescent ALDH substrate, termed BODIPY™‐aminoacetaldehyde (BAAA), and then analysed using flow cytometry. A population of cells with a low side scatter (SSC) and a high level of BAAA staining, termed the SSC lo ALDH br population, was readily discriminated and comprised a mean of 3 ± 5% of leukapheresis samples. A mean of 73 ± 11% of the SSC lo ALDH br population expressed CD34 and 56 ± 25% of all the mobilized CD34 + cells resided within the SSC lo ALDH br population. The SSC lo ALDH br population was largely depleted of cells with mature phenotypes and enriched for cells with immature phenotypes. Sorted SSC lo ALDH br and SSC lo ALDH br CD34 + PBSC were enriched for progenitors with the ability to (1) generate colony‐forming units (CFU) and long‐term culture (LTC)‐derived CFU, (2) expand in primary and secondary LTC, and (3) generate multiple cell lineages. In 21 cancer patients who had undergone autologous PBSC transplantation, the number of infused SSC lo ALDH br cells/kg highly correlated with the time to neutrophil and platelet engraftment ( P < 0·015 and P < 0·003 respectively). In summary, peripheral blood SSC lo ALDH br cells have the phenotypic and functional properties of primitive haematopoietic cells and their number correlates with engraftment following autologous transplantation.