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Endotoxaemia modulates Toll‐like receptors on leucocytes in humans
Author(s) -
Marsik Claudia,
Mayr Florian,
Cardona Francesco,
Derhaschnig Ulla,
Wagner Oswald F.,
Jilma Bernd
Publication year - 2003
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2003.04350.x
Subject(s) - toll , receptor , immunology , toll like receptor , medicine , biology , innate immune system
Summary. The modulation of Toll‐like receptors (TLR) 1, 2 and 4 was studied during experimental human endotoxaemia. Healthy volunteers received 2 ng/kg of lipopolysaccharide (LPS) endotoxin ( n = 10). TLR1, 2 and 4 expression occurred on monocytes and neutrophils, with monocytes expressing higher baseline levels of TLR2. LPS infusion downmodulated TLR4 expression on neutrophils, with maximal downregulation occurring at 24 h (−62% from baseline; P < 0·03 versus baseline). Monocyte TLRs were upregulated in vivo (TLR1 and 2), and in vitro (TLR1, 2 and 4) 8 h after LPS bolus ( P < 0·05 versus baseline). Therefore, neutrophils and monocytes differentially express surface TLRs, and endotoxaemia differentially regulates TLR expression.