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Selective internalization of monoclonal antibodies by B‐cell chronic lymphocytic leukaemia cells
Author(s) -
Sieber Thomas,
Schoeler Dagmar,
Ringel Frauke,
Pascu Maria,
Schriever Folke
Publication year - 2003
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2003.04305.x
Subject(s) - internalization , monoclonal antibody , chronic lymphocytic leukemia , medicine , antibody , monoclonal , immunology , b cell , cancer research , leukemia , receptor
Summary. B‐cell chronic lymphocytic leukaemia (B‐CLL) cannot be cured by conventional chemotherapy, therefore, toxin‐linked therapeutic monoclonal antibodies (mAbs) are increasingly examined for their potential to improve clinical outcome. The current study aimed to identify mAbs that were internalized by the B‐CLL cells of 14 patients, using both flow cytometry and confocal laser scanning microscopy. Anti‐CD5, CD22 and CD40 mAbs were effectively taken up by B‐CLL cells, whereas mAbs against CD19, CD20, CD23 and CD45 were not. This study may form a basis for further research to identify antibodies that may serve as carriers for toxins to treat B‐CLL.