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Chromosome anomalies detected by interphase fluorescence in situ hybridization: correlation with significant biological features of B‐cell chronic lymphocytic leukaemia
Author(s) -
Dewald Gordon W.,
Brockman Stephanie R.,
Paternoster Sarah F.,
Bone Nancy D.,
O'Fallon Judith R.,
Allmer Cristine,
James Charles D.,
Jelinek Diane F.,
Tschumper Renee C.,
Hanson Curtis A.,
Pruthi Rajiv K.,
Witzig Thomas E.,
Call Timothy G.,
Kay Neil E.
Publication year - 2003
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2003.04265.x
Subject(s) - fluorescence in situ hybridization , cd38 , biology , bone marrow , chromosomal translocation , medicine , cytogenetics , pathology , stage (stratigraphy) , oncology , gastroenterology , chromosome , immunology , genetics , gene , cd34 , paleontology , stem cell
Summary. Fluorescence in situ hybridization (FISH) was used to detect 6q–, 11q–, +12, 13q–, 17p– and translocations involving 14q32 in interphase nuclei from blood and/or bone marrow from 113 patients with B‐cell chronic lymphocytic leukaemia (B‐CLL). A total of 87 patients (77%) had a FISH anomaly: 13q– × 1 was most frequent (64%) followed by 13q– × 2 (28%), +12 (25%), 11q– (15%), 17p– (8%) and 6q– (0%). FISH results for blood and bone marrow cells in 38 patients were similar. Purified CD5 + /CD19 + cells from blood were studied in eight patients and results indicate that in some patients not all B cells have FISH anomalies. We used a defined set of hierarchical FISH risk categories to compare FISH results by stable versus progressive disease, age, sex, Rai stage, CD38 + expression and IgV H mutational status. Significant differences in FISH risk distributions were associated with Rai stage, disease status and CD38 + , but not by age, sex or IgV H mutational status. To look for baseline factors associated with high‐risk disease, multivariate analysis of age, sex, Rai stage, CD38 + and disease status versus FISH risk category was performed. Importantly, only CD38 + was significantly associated with high‐risk FISH categories (+12, 11q– and 17p–) after adjustment for the effects of other variables.