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Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase
Author(s) -
Mihara Keichiro,
Imai Chihaya,
CoustanSmith Elaine,
Dome Jeffrey S.,
Dominici Massimo,
Vanin Elio,
Campana Dario
Publication year - 2003
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2003.04217.x
Subject(s) - mesenchymal stem cell , haematopoiesis , cd34 , telomerase reverse transcriptase , telomerase , bone marrow , lymphoblast , immortalised cell line , biology , cell culture , microbiology and biotechnology , cancer research , immunology , stem cell , genetics , gene
Summary. To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT + mesenchymal cells continued to grow for > 2 years; parallel TERT – cultures underwent senescence after 15 weeks. TERT + mesenchymal cells did not form foci in soft agar, had a normal karyotype and could differentiate into osteoblasts and chondrocytes. Their capacity to support leukaemic lymphoblasts and normal CD34 + haematopoietic cells was equal to or greater than that of primary cells; 42 TERT + mesenchymal cell clones varied in their supporting capacity. Immortalized mesenchymal cells offer a promising tool for identifying molecules that regulate human haematopoiesis.