Premium
High levels of hyaluronan production by a malignant lymphoma cell line with primary effusion lymphoma immunophenotype OHK
Author(s) -
Kuwabara Hiroko,
Yoneda Masahiko,
Nagai Masami,
Nishio Hajime,
Tasaka Taizo,
Suzuki Koichi,
Mori Hiroshi
Publication year - 2003
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2003.04206.x
Subject(s) - lymphoma , immunophenotyping , pathology , primary effusion lymphoma , effusion , cell culture , pleural disease , medicine , bcl10 , flow cytometry , biology , cancer research , immunology , respiratory disease , surgery , lung , genetics
Summary. We investigated whether hyaluronan (HA) was produced by a malignant lymphoma cell line with a primary effusion lymphoma immunophenotype, OHK. OHK secreted high levels of HA into the culture supernatant, and reverse transcription polymerase chain reaction showed mRNA for the HA synthases 2 and 3. HA binding protein‐reactive HA was present in the plasma membrane and in the cytoplasm. The inoculation of OHK into severe combined immunodeficiency disease mice produced marked viscid ascites with concentrated HA and a vascular‐rich mesenteric solid tumour. These results show that HA is produced and secreted by the malignant lymphoma cell line OHK.