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Flt3‐ligand induces adhesion of haematopoietic progenitor cells via a very late antigen (VLA)‐4‐ and VLA‐5‐dependent mechanism
Author(s) -
Solanilla Anne,
Grosset Christophe,
Duchez Pascale,
Legembre Patrick,
Pitard Vincent,
Dupouy Maryse,
Belloc Françis,
Viallard JeanFrançois,
Reiffers Josy,
Boiron JeanMichel,
Coulombel Laure,
Ripoche Jean
Publication year - 2003
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2003.04155.x
Subject(s) - homing (biology) , haematopoiesis , integrin , progenitor cell , bone marrow , microbiology and biotechnology , cell adhesion molecule , cell adhesion , chemistry , umbilical vein , adhesion , stem cell , cancer research , immunology , biology , cell , in vitro , biochemistry , ecology , organic chemistry
Summary. The adhesion of haematopoietic progenitor cells (HPC) to the bone marrow microenvironment is a process regulated by cytokines. In this study, we have shown that flt3‐ligand (FL), a growth factor that controls early haematopoiesis, regulated the function and expression of the beta‐1 integrins, very late antigen (VLA)‐4 and VLA‐5 on HPC. The modulation of the adhesiveness of HPC by FL was studied by adhesion assays on umbilical vein endothelial cells (HUVEC). Stimulation by FL induced two peaks of increased adhesiveness of HPC. The first peak was at around 30 min and was mechanistically related to an activation of the beta‐1 integrins, mainly VLA‐4 and VLA‐5. The second peak was at around 12 h and was related to increased expression of VLA‐4 and VLA‐5. The control of HPC adhesiveness by FL is a previously unreported property of FL that may be important for the homing and the retention of flt3‐expressing HPC within the bone marrow microenvironment.