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Hemolink™, an o ‐raffinose cross‐linked haemoglobin‐based oxygen carrier, does not affect activation and function of human platelets in whole blood in vitro
Author(s) -
Leytin Valery,
Mazer David,
Mody Meera,
Garvey Bernadette,
Freedman John
Publication year - 2003
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2003.04068.x
Subject(s) - platelet , whole blood , platelet activation , chemistry , flow cytometry , annexin , andrology , phosphatidylserine , cd63 , in vitro , blood substitute , hemoglobin , immunology , endocrinology , biochemistry , medicine , phospholipid , membrane , microrna , microvesicles , gene
Summary. Haemoglobin‐based oxygen carriers (HBOCs) are anticipated to be safe and efficient alternatives to RBC transfusions. Haemoglobin (Hb) raffimer (Hemolink™; Hemosol, Toronto, ON, Canada) is polymerized human Hb, cross‐linked with o ‐raffinose. As administration of cell‐free Hb may affect blood cells and tissues, this study was focused on evaluating effects of Hb raffimer on human platelets in whole blood in vitro . Citrated blood from healthy donors was incubated with Hb raffimer to achieve raffimer concentrations of 2–50 vol percentage (2–50 g/l). Platelet activation, phosphatidylserine exposure and microparticle generation were measured by flow cytometry. Aperture closure time on collagen/ADP‐ and collagen/epinephrine‐coated membranes was determined by a platelet function analyser (PFA‐100 ® ). We found that addition of Hb raffimer to blood samples up to 50 vol % did not affect human platelets as measured by various markers of platelet activation (CD42b, CD41, PAC‐1, CD62, CD63), procoagulant activity (annexin V) and microparticle formation; differences between Hb raffimer‐ and lactated Ringer's‐diluted blood were not significant. Similarly, no adverse effect of Hb raffimer on closure time was observed at concentrations up to 50 vol %, in comparison with Ringer's solution. These data indicate that exposure of human blood to high concentrations of Hb raffimer in vitro did not cause platelet activation nor affect platelet function.