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The naive T‐lymphocyte compartment is well preserved in patients with chronic myelogenous leukaemia in chronic phase
Author(s) -
Isoda Atsushi,
Yokohama Akihiko,
Matsushima Takafumi,
Tsukamoto Norifumi,
Nojima Yoshihisa,
Karasawa Masamitsu
Publication year - 2002
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2002.03943.x
Subject(s) - immunology , cd8 , naive t cell , cd28 , t lymphocyte , phenotype , biology , lymphocyte , t cell , medicine , immune system , t cell receptor , genetics , gene
Summary. In chronic myelogenous leukaemia (CML), clonal change occurs in all myeloid and B‐cell lineages, but very rarely T‐cell lineages. A detailed three‐colour cytometric analysis of peripheral lymphocytes was performed in 22 patients with chronic‐phase CML (CP‐CML). CD45 gating analysis was used to discriminate between lymphocytes and basophils. The peripheral lymphocyte pool was comprised of a significant proportion of naive CD4 cells, defined by a CD4 + 45RA + phenotype [47·0 ± 19·6% (mean ± SD) of the total CD4 + cells], and naive CD8 cells, defined by a CD8 + CD45RA + CD28 + phenotype (35·1 ± 19·7% of total CD8 + cells), even in patients with long disease duration. The percentage of CD8 naive T cells showed inverse correlation with age, whereas no correlation was observed with disease duration. Possible explanations for the preservation of naive lymphocytes include (1) that the naive T cells differentiated from co‐existing normal stem cells or (2) that long‐lived naive T cells persisted from the CML onset and expanded peripherally (thymus independent). Either mechanism or a combination of both mechanisms might contribute to maintaining the naive compartment size.