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BDCM: a novel B‐cell line with genetic and functional similarity to dendritic cells
Author(s) -
Kharbanda Sandhya,
Salter Russell D.,
Dong Xin,
TumaWarrino Renee J.,
Steinman Richard A.
Publication year - 2002
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2002.03908.x
Subject(s) - priming (agriculture) , dendritic cell , autocrine signalling , monocyte , cell culture , cytokine , biology , myeloid , microbiology and biotechnology , immunology , transcription factor , t cell , antigen , genetics , immune system , gene , botany , germination
Summary. We describe a B‐cell line, BDCM, which arose spontaneously from culture of a pheresis product from a patient with M5a myeloid leukaemia. Cell growth was associated with autocrine activation of the s ignal t ransducer and a ctivator of t ranscription 1 (Stat‐1) transcription factor. Although the cells expressed several B‐cell surface markers and had a rearranged immunoglobin J region, they also exhibited several characteristics associated with dendritic cells. These included extensive surface projections, cross‐priming ability and strong T cell‐stimulating capability. In addition, the cytokine production profile of BDCM cells was nearly identical to that of mature monocyte‐derived dendritic cells.