Premium
Dominant expression of interleukin 10 but not interferon γ in CD4 – CD8 – αβT cells of autoimmune lymphoproliferative syndrome
Author(s) -
Ohga Shouichi,
Nomura Akihiko,
Takahata Yasushi,
Ihara Kenji,
Takada Hidetoshi,
Wakiguchi Hiroshi,
Kudo Yuji,
Hara Toshiro
Publication year - 2002
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2002.03848.x
Subject(s) - cd8 , interferon gamma , microbiology and biotechnology , cytotoxic t cell , cytokine , interferon , cd3 , interleukin 4 , biology , immunology , lymphoproliferative disorders , immune system , lymphoma , in vitro , biochemistry
Summary. Cytokine expression in CD4 – CD8 – double‐negative (DN) T cells of autoimmune lymphoproliferative syndrome (ALPS) was analysed. Two patients with DN αβT‐cell expansion showed higher serum interleukin 10 (IL‐10) levels than one patient without it. Intracellular flow‐cytometric analysis indicated the IL‐10‐expressing CD3 + CD4 – CD8 – cells in patients with lymphoproliferation. Quantitative real‐time polymerase chain reaction revealed ∼100 times higher IL‐10, but not interferon‐γ or transforming growth factor‐β in DN than in single‐positive T cells. IL‐10 was exclusively expressed in DN αβ but not γδΤ cells. Circulating DN αβT cells may constitutively express IL‐10 and contribute to the ALPS phenotype.